Journal: Frontiers in Endocrinology

Article Title: Association of angiotensin II and receptors in peri-implantation endometrium with microvessel density and pregnancy outcomes of women with recurrent implantation failure after embryo transfer

doi: 10.3389/fendo.2023.1206326

Figure Lengend Snippet: Ang II, AT1R, and AT2R expression in endometrium between fertile controls, RM and RIF. (A) Representative images of immunohistochemical staining of Ang II, AT1R, and AT2R among groups. LE, luminal epithelium; GE, glandular epithelium; ST, stroma cells. Scale bar=100 μm. (B) Corresponding quantitative analysis. Data are presented as mean ± SEM for AngII, AT1R, and AT2R and median (range) for stromal AT1R/AT2R expression ratios in all compartments of endometrium. *p < 0.05, ***p<0.001, NS, not significant.

Article Snippet: The slides were washed in PBS and incubated in blocking solution (5% goat serum albumin in PBS) for 1 h at room temperature, and incubated overnight at 4°C with primary antibody, either rabbit anti-human Ang II polyclonal antibody (PA5-33339, Thermo Fisher, US) at 1:400 dilution, rabbit anti-human AT1R polyclonal antibody (AAR-011, Alomone Labs, Israel) at 1:1500 dilution, rabbit anti-human AT2R antibody (ab19134, AbCam, UK) at 1:150 dilution, or rabbit polyclonal anti-human vWF antibody (A0082, DAKO, Japan) at 1:1000 dilution.

Techniques: Expressing, Immunohistochemical staining, Staining